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Literature summary extracted from

  • Tateno, T.; Okada, Y.; Tsuchidate, T.; Tanaka, T.; Fukuda, H.; Kondo, A.
    Direct production of cadaverine from soluble starch using Corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase (2009), Appl. Microbiol. Biotechnol., 82, 115-121.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.1 biotechnology coexpressed in a construct in Corynebacterium glutamicum, combined with Escherichia coli K-12 lysine decarboxylase for an one-step production of cadaverine, from soluble starch and the yield of cadaverine is 23.4 mM after 21 h. Construction of the Escherichia coli-Corynebacterium glutamicum shuttle vector, producing lysine decarboxylase under the control of the high constitutive expression promotor, and transformed this vector into Corynebaterium glutamicum. The engineered Corynebacterium glutamicum expresses both lysine decarboxlase and alpha-amylase, which retains their activity Streptococcus equinus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 coexpressed in a construct in Corynebacterium glutamicum, combined with Escherichia coli K-12 lysine decarboxylase for an one-step production of cadaverine. Construction of the Escherichia coli-Corynebacterium glutamicum shuttle vector, producing lysine decarboxylase under the control of the high constitutive expression promotor, and transformed this vector into Corynebaterium glutamicum. The engineered Corynebacterium glutamicum expresses both lysine decarboxlase and alpha-amylase, which retains their activity Streptococcus equinus
4.1.1.18 expressed in Corynebacterium glutamicum ATCC 13032 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.18 82000
-
 SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Streptococcus equinus
-
 coexpressed in a construct in Corynebacterium glutamicum, combined with Escherichia coli K-12 lysine decarboxylase for an one-step production of cadaverine
-
4.1.1.18 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.18 L-Lys
-
 Escherichia coli Cadaverine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 AmyA
-
 Streptococcus equinus
4.1.1.18 CadA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.18 pyridoxal 5'-phosphate
-
 Escherichia coli